Journal:
Article Title: Rab8 Regulates Basolateral Secretory, But Not Recycling, Traffic at the Recycling Endosome
doi: 10.1091/mbc.E07-09-0902
Figure Lengend Snippet: Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G tsO45 released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.
Article Snippet: We are especially indebted to Agnes Ang, and Ira Mellman (both of Genentech) for providing the dsRFP-Rab8 constructs as well as the YFP-VSV-G tsO45 mutant construct.
Techniques: Synthesized, Control, Labeling, Expressing
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